ART Database

This family of proteins is found in bacteria, archaea and eukaryotes. DarT acting as the toxin in a toxin-antitoxin (TA) system. It is an enzyme that specifically modifies thymidines on single-stranded DNA in a sequence-specific manner by a nucleotide-type modification called ADP-ribosylation. This modification can be removed by DarG, the antitoxin macrodomain protein.

Proteins containing this domain can be found in bacteria, archaea and eukaryotes. Proteins containing this domain are distantly similar to (INTERPRO). Studies in search for novel ADP-ribosylation systems in bacterial genomes have identified an operon that encodes a conserved protein containing a distinct type of macrodomain associated with this domain. This led to the identification of a toxin-antitoxin (TA) system, with DarT acting as the toxin. It is an enzyme that specifically modifies thymidines on single-stranded DNA in a sequence-specific manner by a nucleotide-type modification called ADP-ribosylation. This modification in turn can be removed by DarG, the antitoxin macrodomain protein. In addition, it was illustrated that substitution of the single completely conserved glutamate residue resulted in attenuation of function where DarT was non-toxic [PUBMED:27939941].

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Source: Pfam 34.0, rp75 Number of sequences: 807 Average length of the domain: 180 aa HMM: Model length: 200 Clustering level: 80% Alignment: ClustalO Additional information: sequences longer than 50 amino acids